NGS Tests

NGS:

Next-generation sequencing (NGS), massively parallel or deep sequencing are related terms that describe a DNA sequencing technology which has revolutionised genomic research. Using NGS an entire human genome can be sequenced within a single day.

In contrast, the previous Sanger sequencing technology used to decipher the human genome, required over a decade to deliver the final draft. Although in genome research NGS has mostly superseded conventional Sanger sequencing, it has not yet translated into routine clinical practice.

Advantage of NGS:

NGS can be used to analyse DNA and RNA samples and is a popular tool in functional genomics. In contrast to microarray methods, NGS-based approaches have several advantages including:

  • Prior knowledge of the genome or genomic features is not required
  • It offers single-nucleotide resolution, making it possible to detect related genes (or features), alternatively spliced transcripts, allelic gene variants and single nucleotide polymorphisms
  • The higher dynamic range of signal
  • Requires less DNA/RNA as input (nanograms of materials are sufficient)
  • Higher reproducibility

NGS Technologies:

  • Illumina (Solexa) sequencing: Illumina sequencing works by simultaneously identifying DNA bases, as each base emits a unique fluorescent signal, and adding them to a nucleic acid chain
  • Roche 454 sequencing: This method is based on pyrosequencing, a technique which detects pyrophosphate release, again using fluorescence, after nucleotides are incorporated by polymerase to a new strand of DNA.
  • Ion Torrent: Proton / PGM sequencing: Ion Torrent sequencing measures the direct release of H+ (protons) from the incorporation of individual bases by DNA polymerase and therefore differs from the previous two methods as it does not measure light.
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List of NGS Test collection centres in India is Mumbai, Delhi, Bangalore, Hyderabad, Ahmedabad, Chennai, Kolkata, Surat, Pune, Jaipur, Visakhapatnam, Kanpur, Nagpur, Lucknow, Thane, Bhopal, Indore, Pimpri-Chinchwad, Patna, Vadodara, Ghaziabad, Ludhiana, Agra, Nashik, Faridabad, Meerut, Rajkot, Kalyan-Dombivli, Vasai-Virar, Varanasi, Srinagar, Aurangabad, Dhanbad, Amritsar, Navi, Mumbai, Allahabad, Howrah, Ranchi, Gwalior, Jabalpur, Coimbatore, Vijayawada, Jodhpur, Madurai, Raipur, Chandigarh, Guntur, Guwahati, Solapur, Hubli–Dharwad, Mysore, Tiruchirappalli, Bareilly, Moradabad, Tiruppur, Gurgaon, Aligarh, Jalandhar, Bhubaneswar, Salem, Mira-Bhayandar, Warangal, Jalgaon, Kota, Bhiwandi, Saharanpur, Gorakhpur, Bikaner, Amravati, Noida, Jamshedpur, Bhilai, Cuttack, Firozabad, Kochi, Nellore, Bhavnagar, Dehradun, Durgapur, Asansol, Rourkela, Nanded, Kolhapur, Ajmer, Akola.