Cell Line Authentication DNA Test

Cell Line Authentication DNA Test

Disease: Genetics

Method: STR DNA Technology

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6,500.00/- Rs ₹8,500.0023% off

  • Results in : 10-12 days

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Cell Line Authentication DNA Test Cost 6500 Rs


Test Name Cell Line Authentication DNA Test
Test type Research
Pre-test Information Extracted DNA- should be eluted in low TE buffer or Nucleus Free water. 10-100 ng/µl volume- 10-20 µl Transport precaution- The sample should be collected in a microcentrifuge tube and frozen at Minus 20 °C and should be transported with an ice pack. Cell Pellets- 1 x 106 cells/ml Transport precaution- Freeze it at -80 °C and pack with dry ice. Spot on FTA- follow the standard guidelines for sample preparation, then spot 20 µl sample on the card. Transport precaution- single FTA card packed in a single zip bag don’t mix 2 FTA cards in a single zip bag. Tissue or tumor cells- size should be approx 5 mg. Transport precaution- transport it with an ice pack.
Report Delivery 10-12 days
Components Reference sample comparison to the ATCC database and DSMZ Databse for identity confirmation
Price ₹ 6500
Method STR DNA Technology

Cell Line Authentication DNA Test Details


Short Description

DNA Labs India offers human cell line identification services. DNA Labs India provides reliable, fast and affordable authentication of human cancer cell lines, stem cell lines, xenografts, and more utilizing STR DNA technology current clients include major research and cancer institutions, pharmaceutical and biotechnology companies within India and throughout the world. • 17 highly polymorphic markers as genetic markers to establish identity and confirm authenticity • Exceeds the ASN-0002 guidelines of 8 loci • Suitable for use with highly fragile human embryonic stem cells (hES)

Test Specifications

  • Speciality: Research

  • Components: Reference sample comparison to the ATCC database and DSMZ Databse for identity confirmation

  • Department: Forensics

  • Shipping Stability: Room Temperature

PreTest Information

Extracted DNA- should be eluted in low TE buffer or Nucleus Free water. 10-100 ng/µl volume- 10-20 µl Transport precaution- The sample should be collected in a microcentrifuge tube and frozen at Minus 20 °C and should be transported with an ice pack. Cell Pellets- 1 x 106 cells/ml Transport precaution- Freeze it at -80 °C and pack with dry ice. Spot on FTA- follow the standard guidelines for sample preparation, then spot 20 µl sample on the card. Transport precaution- single FTA card packed in a single zip bag don’t mix 2 FTA cards in a single zip bag. Tissue or tumor cells- size should be approx 5 mg. Transport precaution- transport it with an ice pack.

Detail Description

Cell Line Authentication DNA Test Cost in India: Symptoms and Diagnosis

Cell line authentication is an important process in the field of biology and medicine. It involves the verification of the identity and purity of cell lines used in research and clinical trials. DNA Labs India offers cell line authentication DNA tests at an affordable cost of INR 6500. In this blog, we will discuss the symptoms and diagnosis of cell line authentication.

Symptoms of Cell Line Authentication

Cell line authentication is necessary to ensure the accuracy and reliability of research and clinical trials. The use of misidentified or contaminated cell lines can lead to incorrect data, wasted resources, and even harm to patients. Symptoms of incorrect cell line authentication include:

  • Inconsistent results
  • Unreliable data
  • Delayed research progress
  • Wasted resources
  • Inaccurate clinical trial outcomes
  • Potential harm to patients

Diagnosis of Cell Line Authentication

Cell line authentication DNA testing is the most accurate and reliable method for verifying the identity and purity of cell lines. The test involves comparing the DNA profile of the cell line with a known reference sample. The reference sample can be from the original donor or from a reliable cell bank. The test can detect misidentified or contaminated cell lines, as well as genetic changes that may have occurred during cell culture. DNA Labs India offers cell line authentication DNA testing at an affordable cost of INR 6500.

In conclusion, cell line authentication is an important process in the field of biology and medicine. It ensures the accuracy and reliability of research and clinical trials. DNA Labs India offers cell line authentication DNA testing at an affordable cost of INR 6500. Symptoms of incorrect cell line authentication include inconsistent results, unreliable data, delayed research progress, wasted resources, inaccurate clinical trial outcomes, and potential harm to patients. The most accurate and reliable method for verifying the identity and purity of cell lines is through DNA testing.

Contact DNA Labs India today to schedule a cell line authentication DNA test and ensure the accuracy and reliability of your research and clinical trials.

Frequently Asked Questions

  • What is the cost of Cell Line Authentication DNA Test?

    Cost of Cell Line Authentication DNA Test is 6500 Rs

  • What is Cell Line Authentication?

    Tissue culture cells are important tools in many laboratories. Unfortunately, many cell lines are misidentified or contaminated with other cells, potentially invalidating published data and wasting time and laboratory resources. 

  • When should cell line authentication be conducted?

    Authentication is required every time a new line is established or acquired. Cells should be authenticated again before freezing, once every two months that the culture is actively growing, at any point that the performance of the line is inconsistent or yields unexpected results, and before publication.

  • How does it work?

    The number of repeats in STR loci can be highly variable among individuals, which make these genetic markers effective for human identification or cell line authentication purposes. STRs have become popular DNA repeat markers because they are easily amplified by the polymerase chain reaction (PCR) using PCR primers that bind to the flanking regions surrounding the STR repeat. 

  • Cell Line Authentication and Characterization Methods

    Cell line characterization is performed for Master Cell Banks (MCB), Working Cell Banks (WCB), and End-of-Production Cells (EPC) or cells at the limit (CAL) of in vitro cell age used for production. According to current regulations, the following aspects must be considered:

    • The origin and history of the cell line cellular morphology and growth characteristics
    • Cellular identity Purity of the cell lines (i.e., absence of contaminating cells, microbial contaminations, and contaminations by adventitious viruses)
    • Tumorigenicity and oncogenicity Stability 
  • Cell Line Characterization

    Proper cell line characterization assays are crucial to confirm the certainty of contaminant-free cell banks. Extensive cell line authentication, including phenotypic or genotypic identity testing, is required for mammalian, microbial, and insect cell substrates testing when working to generate biopharmaceuticals. 

  • Authentication of Nonhuman Cell Lines

    When considering authentication of nonhuman cell lines, the extensive body of technical knowledge on STR profiling, which is captured in the ANSI standard and by other organizations (e.g., The International Society for Forensic Genetics), and the proven record of practicality and fit-for-purpose of STR profiling, make it an obvious choice for consideration as a standard method for nonhuman cell lines.

  • What is the cost of Cell Line Authentication?

    The cost of Cell Line Authentication is 6500 Rs

  • How many days does it take for reports?

    Reports will take 8-10 working days

₹6,500.00 ₹8,500.0023% off

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