Overview
16S rRNA Sequencing is a molecular diagnostic technique that identifies bacterial species by analyzing the 16S ribosomal RNA gene—a genetic marker present in all bacteria. This gene contains both conserved regions shared across bacterial species and hypervariable regions unique to each species, making it an ideal target for precise bacterial identification. Unlike traditional culture methods that require bacteria to grow on artificial media, 16S sequencing detects bacterial DNA directly from clinical specimens, enabling identification of slow-growing, fastidious, and unculturable organisms. DNA Labs India offers this advanced test using state-of-the-art next-generation sequencing platforms in our ISO 9001:2015 certified laboratory. The test is invaluable for diagnosing culture-negative infections, identifying pathogens in fever of unknown origin, analyzing deep-seated infections, and characterizing the human microbiome for research purposes. With free home sample collection available across India and results delivered within two weeks, DNA Labs India makes this cutting-edge genomic technology accessible to clinicians and researchers nationwide. All results are interpreted by our team of microbiologists and bioinformaticians to ensure clinical relevance and actionable insights for patient care.
Provides paired-end 150 bp Next-Generation Sequencing (NGS) services using the Illumina MiSeq platform for customers who need DNA sequencing data for research or other sequencing applications.
The primary purpose of 16S rRNA Sequencing is to identify bacterial species present in a clinical or research sample by analyzing the 16S ribosomal RNA gene. This test is specifically designed to detect bacteria that are difficult or impossible to grow using standard culture methods, including slow-growing, fastidious, and obligate intracellular organisms. It serves as a critical diagnostic tool for culture-negative infections, fever of unknown origin, and deep-seated infections where rapid and accurate pathogen identification is essential for targeted therapy. Additionally, it is widely used in microbiome research to characterize the bacterial composition of various body sites, including the gut, skin, and oral cavity, supporting studies in inflammatory bowel disease, irritable bowel syndrome, and other microbiome-associated conditions.
Methodology
How the work is done
Customer submits an enquiry
Sample requirements and sequencing specifications are confirmed.
Extracted DNA sample is submitted.
DNA sample undergoes MiSeq sequencing.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: Results are typically available within 2 weeks of sample receipt at the laboratory. You will be notified via your preferred communication channel (online portal, email, or WhatsApp) once the report is ready.
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | Results are typically available within 2... | ₹5,000 | per sample |
Not included: DNA extraction. · Clinical diagnosis or interpretation. · Genetic counselling. · Additional bioinformatics analysis, unless separately offered.
Applications
Who orders this service
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
Is fasting required before providing a sample for 16S rRNA sequencing?
No, fasting is not required for 16S rRNA sequencing. However, please follow any specific instructions provided by your doctor regarding sample collection, such as using sterile containers for urine or fluid samples.
How is 16S rRNA sequencing different from a standard bacterial culture?
A standard culture attempts to grow bacteria on a plate, but many bacteria are difficult or impossible to grow in a lab. 16S sequencing detects bacterial DNA directly from the sample, identifying both culturable and unculturable bacteria, providing a more complete picture of the infection.
What kind of samples can be sent for 16S rRNA sequencing?
We accept a wide variety of samples, including extracted DNA, body fluids (CSF, synovial fluid, pleural fluid), pus, tissues, stool, and swabs from infection sites. Please contact us to confirm sample suitability for your specific case.
How accurate is 16S rRNA sequencing?
The test is highly accurate for bacterial identification. When performed in an ISO 9001:2015 certified laboratory with proper bioinformatics analysis, it can identify bacteria with a high degree of precision, often resolving cases where standard cultures are negative.
How do I book a 16S rRNA sequencing test?
You can book directly on this page by clicking the 'Book Now' button, or by calling our 24/7 customer support number. We will coordinate free home sample collection and answer any pre-test questions you may have.
Will the report tell me which antibiotic to use?
16S rRNA sequencing identifies the bacteria present in the sample. While it can sometimes inform treatment decisions based on the species identified, it is not a substitute for formal antibiotic susceptibility testing (AST). For antibiotic choices, this test is often used in conjunction with clinical assessment and other laboratory findings.
Is my sample and data kept private?
Yes, 100%. DNA Labs India adheres to strict confidentiality protocols. Your genetic data and personal information are securely stored and never shared without your explicit consent, in compliance with applicable data protection regulations.
Can this test be used for research purposes?
Absolutely. 16S sequencing is a cornerstone of microbiome research. We offer specialized services for research cohorts, including detailed bioinformatics analysis of microbial communities. Please contact our research support team for customized project quotes.
How long are the results valid?
The result is a snapshot of the bacterial DNA present in the sample at the time of collection. For diagnostic purposes, it directly informs immediate clinical management. For research, the data serves as a permanent record of that sample's bacterial composition.
What is the difference between 16S rRNA and Whole Genome Sequencing (WGS)?
16S sequencing targets a single gene to identify which bacteria are present. It is faster and more cost-effective for bacterial community analysis. Whole Genome Sequencing sequences all the DNA in a sample, providing deeper functional insights including resistance genes, but at a higher cost and complexity.
Can 16S rRNA sequencing detect antibiotic resistance genes?
16S rRNA sequencing is primarily designed for bacterial identification, not resistance gene detection. While the identified species may suggest known resistance patterns, formal antibiotic resistance profiling requires Whole Genome Sequencing or phenotypic susceptibility testing. Consult your physician for comprehensive resistance evaluation.
Is this test suitable for pediatric patients?
Yes, 16S rRNA sequencing can be performed on samples from patients of all ages, including pediatric patients. The test analyzes bacterial DNA from the submitted sample and does not require age-specific protocols. Sample collection in children should be performed by a trained pediatric healthcare professional.
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