Overview
Bacterial 16S rRNA V3-V4 amplicon sequencing is a cutting-edge molecular diagnostic technique used to identify and characterize bacterial communities in clinical samples. The 16S rRNA gene is highly conserved among bacteria but contains variable regions (V1-V9) that allow species-level discrimination. The V3-V4 region is particularly informative due to its high variability, enabling differentiation of closely related species. This test employs next-generation sequencing (NGS) to amplify and sequence the V3-V4 region, followed by bioinformatics analysis to compare sequences against reference databases. It is especially useful for detecting bacteria that are difficult to culture, slow-growing, or unculturable, and for analyzing polymicrobial infections. The test provides a comprehensive profile of the bacterial microbiome, aiding in accurate diagnosis, targeted therapy, and infection control. At DNA Labs India, we offer this advanced test at a competitive price of INR 10,000, with free home sample collection across major cities. The test is performed on extracted DNA from various clinical specimens, ensuring high sensitivity and specificity. Results are typically available within 8 weeks, and our expert team provides a detailed interpretive report to guide clinical decisions.
To be completed — reclassified from Tests on 2026-11-13.
The primary purpose of this test is to identify bacterial species present in a clinical sample by analyzing the 16S rRNA gene's V3-V4 region. It helps in diagnosing bacterial infections, especially when conventional culture methods fail or are inconclusive. The test also provides insights into the bacterial community structure, which can be crucial for understanding polymicrobial infections, monitoring treatment response, and guiding antimicrobial stewardship.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: Results are typically available within 8 weeks from sample receipt. You will be notified via email/SMS when your report is ready.
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | Results are typically available within 8... | ₹10,000 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is the cost of Bacterial 16S rRNA V3-V4 Amplicon Sequencing and Analysis?
The cost is INR 10,000 per sample at DNA Labs India. This includes sample collection, sequencing, bioinformatics analysis, and a detailed report.
What is the sample type required for this test?
The test requires extracted DNA from a clinical specimen. The DNA extraction is performed at our laboratory from samples such as tissue, fluid, or swabs.
How long does it take to get results?
The turnaround time is approximately 8 weeks from the date of sample receipt.
Is fasting required before the test?
No, fasting is not required for this test.
Can this test detect all types of bacteria?
It can detect most bacteria, including those that are difficult to culture. However, some species may not be identified at the species level due to database limitations.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings across major cities in India.
What is the difference between 16S rRNA V3-V4 sequencing and full 16S rRNA sequencing?
V3-V4 sequencing targets a specific variable region, which is cost-effective and sufficient for most bacterial identifications. Full gene sequencing provides more resolution but is more expensive and time-consuming.
Can this test determine antibiotic resistance?
No, this test does not provide antibiotic susceptibility information. Additional tests are needed for resistance profiling.
What should I do if my symptoms persist after the test?
Consult your healthcare provider for further evaluation. The test results should be interpreted in conjunction with your clinical history.
Are there any risks associated with the test?
The test is safe. The only risk is minimal discomfort or bleeding at the sample collection site, similar to routine blood tests.
How should I prepare for the test?
No special preparation is needed. Inform your doctor about any recent antibiotic use, as it may affect the results.
Is the test covered by insurance?
Insurance coverage varies. We recommend checking with your insurance provider. We also offer affordable self-pay options.
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