Overview
Bacterial genome de novo assembly and annotation is a comprehensive genomic analysis that determines the complete DNA sequence of a bacterium and identifies its functional elements. Using Illumina's high-throughput sequencing technology, this test provides a high-resolution view of the bacterial genome, enabling researchers and clinicians to understand genetic determinants of pathogenicity, antimicrobial resistance, and evolutionary relationships. The process involves isolating bacterial DNA, sequencing it to generate millions of short reads, assembling these reads into contiguous sequences (contigs), and annotating the genome to locate genes, regulatory regions, and other features. This information is crucial for outbreak investigations, vaccine development, and personalized treatment strategies. At DNA Labs India, we offer this advanced test at a competitive price of Rs 18000, with free home sample collection across major cities. Our state-of-the-art bioinformatics pipeline ensures accurate and reliable results, supported by expert geneticists and infectious disease specialists.
To be completed — reclassified from Tests on 2026-11-13.
The primary purpose of bacterial genome de novo assembly and annotation is to obtain a complete and accurate genetic blueprint of a bacterial isolate. This is essential for: 1) Identifying the exact species and strain of bacteria, especially in cases where conventional methods are inconclusive. 2) Detecting antimicrobial resistance genes, guiding effective antibiotic therapy. 3) Understanding virulence factors and pathogenic mechanisms. 4) Conducting epidemiological surveillance and outbreak tracking. 5) Supporting research in bacterial evolution and comparative genomics. 6) Facilitating the development of novel diagnostics, vaccines, and therapeutics. By providing a comprehensive genomic map, this test empowers clinicians and researchers with actionable insights for improved patient outcomes and public health interventions.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: Reports are typically available within 4 weeks from the date of sample receipt.
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | Reports are typically available within 4... | ₹18,000 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is bacterial genome de novo assembly and annotation?
It is a process that determines the complete DNA sequence of a bacterium and identifies all its functional elements, such as genes, regulatory regions, and resistance markers. This is done by sequencing the DNA, assembling the reads into a complete genome, and annotating the genome to locate genes and other features.
What is the cost of this test at DNA Labs India?
The test costs Rs 18000, which includes DNA extraction, library preparation, Illumina sequencing, de novo assembly, annotation, and a detailed report. Free home sample collection is available for online bookings.
What is the turnaround time for results?
Reports are typically delivered within 4 weeks from the date of sample receipt.
What sample is required for this test?
A pure bacterial culture isolate (1-2 colonies) or a clinical specimen such as blood, sputum, or urine, as advised by your physician. The sample should be collected in a sterile container.
Is fasting required before the test?
No, fasting is not required for this test.
Can this test detect antimicrobial resistance?
Yes, the annotation identifies genes associated with antimicrobial resistance, which can guide treatment decisions. However, results should be correlated with phenotypic susceptibility testing.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings across major cities in India, including Mumbai, Delhi, Bangalore, Hyderabad, and many more.
How should the sample be transported?
The sample should be transported to the lab as soon as possible. For culture isolates, keep at 2-8°C. For clinical specimens, maintain cold chain if transport exceeds 2 hours.
What is the difference between de novo assembly and reference-based assembly?
De novo assembly builds a genome from scratch without a reference, making it ideal for novel organisms. Reference-based assembly maps reads to a known reference genome, which is faster but may miss novel sequences.
Can this test be used for outbreak investigations?
Yes, the high-resolution genomic data can be used for epidemiological tracking, identifying transmission routes, and understanding outbreak dynamics.
Are there any risks associated with this test?
No significant risks are associated with this test. If a blood sample is taken, there is a minimal risk of bruising or infection at the puncture site.
How should I interpret the results?
Results should be interpreted by a qualified healthcare professional. The report includes information on genome quality, gene content, resistance genes, and virulence factors, which can be used for clinical and research purposes.
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