Overview
Bacterial genome de novo assembly and annotation is a comprehensive genomic analysis that determines the complete DNA sequence of a bacterial isolate and identifies its functional elements. DNA Labs India utilizes a hybrid approach combining Illumina short-read sequencing (high accuracy) and Oxford Nanopore Technology (ONT) long-read sequencing (contiguity) to generate a high-quality, complete genome assembly. This method enables precise identification of bacterial species, strain typing, detection of antimicrobial resistance genes, virulence factors, and phylogenetic relationships. The service is invaluable for clinical diagnostics, epidemiological surveillance, and research applications. The test is performed on a pure bacterial culture isolate, and the turnaround time is 4 weeks. The cost is INR 45000, inclusive of sequencing, bioinformatics analysis, and a detailed interpretative report.
To be completed — reclassified from Tests on 2026-11-13.
The purpose of this test is to provide a complete and accurate genomic characterization of a bacterial isolate. It is used to identify bacterial strains that are difficult to culture or identify by conventional methods, to determine antimicrobial resistance profiles, to investigate outbreaks, and to support epidemiological studies. The hybrid assembly ensures high-quality genome sequences, enabling reliable annotation of genes and functional elements.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: Reports are typically available within 4 weeks after the sample is received.
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | Reports are typically available within 4... | ₹45,000 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is bacterial genome de novo assembly?
It is the process of reconstructing the complete genome sequence of a bacterium from sequencing reads without a reference genome. This provides a comprehensive genetic blueprint of the organism.
Why use hybrid sequencing (Illumina + ONT)?
Hybrid sequencing combines the high accuracy of Illumina short reads with the long-read capability of ONT, resulting in a complete, accurate genome assembly that is ideal for identifying resistance genes, virulence factors, and strain types.
What sample is required for this test?
A pure bacterial culture isolate (1-2 isolated colonies or 1 mL of liquid culture) is required. The sample must be collected and transported in a sterile container with appropriate transport medium.
How long does it take to get results?
The turnaround time is approximately 4 weeks from the time the sample is received at the laboratory.
What is the cost of the test?
The test costs INR 45000, which includes sequencing, bioinformatics analysis, and a detailed report.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings across major cities in India.
Can this test detect antimicrobial resistance?
Yes, the test identifies known antimicrobial resistance genes in the bacterial genome, which helps predict resistance to specific antibiotics.
Is this test useful for outbreak investigations?
Absolutely. The high-resolution genomic data allows for precise strain typing and phylogenetic analysis, which is essential for tracking outbreaks and understanding transmission.
What is the difference between de novo assembly and re-sequencing?
De novo assembly builds a genome from scratch without a reference, while re-sequencing maps reads to a known reference. De novo is essential for novel or divergent organisms.
Are there any risks to the patient?
No, the test is performed on a bacterial culture, so there are no direct risks to the patient.
How should the sample be transported?
The sample should be transported at room temperature or refrigerated (2-8°C) and must reach the laboratory within 24-72 hours. Do not freeze.
Will the report include interpretation?
Yes, the report includes a detailed interpretation by clinical geneticists and infectious disease specialists, including clinical implications.
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