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Sequencing service

Bacterial Genome Sequencing, De Novo Assembly and Annotation - Illumina and ONT Hybrid

Reviewed for accuracy
₹125,000
Per sample
final quote on confirmation
Reports are typically available wi...
Turnaround
from sample receipt
Extracted DNA
Sample
input material
Enquiry
How to order
no payment online

Overview

Bacterial genome sequencing is a comprehensive molecular technique that determines the complete DNA sequence of a bacterial organism. This test employs a hybrid approach combining Illumina short-read sequencing, known for high accuracy, and Oxford Nanopore Technologies (ONT) long-read sequencing, which facilitates assembly across repetitive regions. The hybrid strategy leverages the strengths of both platforms to produce a high-quality, complete genome assembly. De novo assembly reconstructs the genome without a reference, while annotation identifies genes, functional elements, and potential virulence or resistance determinants. This service is particularly valuable for research, epidemiological surveillance, and clinical diagnostics when traditional methods are inconclusive. At DNA Labs India, we provide end-to-end solutions from DNA extraction to bioinformatics analysis, ensuring reliable results for clinicians and researchers.

To be completed — reclassified from Tests on 2026-11-13.

The purpose of this test is to obtain a complete and accurate bacterial genome sequence for detailed genetic analysis. It aids in understanding bacterial biology, identifying antimicrobial resistance genes, tracking outbreaks, and characterizing virulence factors. In clinical settings, it can be used for definitive diagnosis of infections when conventional tests fail, and for guiding targeted therapy in complex cases.

Sample requirements

What to send us

Sample type: Extracted DNA
Volume: ≥1 µg (concentration ≥50 ng/µL)
Container: Sterile microcentrifuge tube
Collection: DNA extraction from culture or clinical specimen

Deliverables & turnaround

What you receive

Turnaround: Reports are typically available within 8 weeks from the date of sample receipt.

Pricing

Transparent pricing

OptionTurnaroundPrice (INR)Unit
StandardReports are typically available within 8...₹125,000per sample

Limitations & precautions

What this service cannot do

Requires high-quality extracted DNA; poor quality may lead to incomplete assembly
Hybrid approach is more expensive than single-platform methods
Annotation accuracy depends on existing databases
Not a routine diagnostic test; reserved for complex cases
Turnaround time is longer (8 weeks) compared to standard tests

Questions customers ask

Frequently asked questions

What is the difference between Illumina and ONT sequencing?

Illumina produces short reads with high accuracy, while ONT produces long reads that help in assembling repetitive regions. The hybrid approach combines both for optimal results.

Why is de novo assembly important?

De novo assembly reconstructs the genome without a reference, allowing detection of novel sequences and structural variations.

How long does it take to get results?

The turnaround time is approximately 8 weeks due to the complexity of sequencing and analysis.

What type of sample is required?

Extracted DNA from a bacterial culture or clinical specimen is required. The laboratory will provide instructions for submission.

Can this test detect antimicrobial resistance?

Yes, the sequencing data is analyzed for the presence of known antimicrobial resistance genes.

Is this test covered by insurance?

Typically, this test is not covered by insurance as it is often used for research or complex diagnostic cases. Please check with your provider.

What is the cost of the test?

The cost is Rs 125000.0, which includes sequencing, assembly, annotation, and bioinformatics analysis.

Do I need to fast before the test?

No, fasting is not required as the test is performed on extracted DNA.

Can home sample collection be arranged?

Yes, we offer free home sample collection for online bookings across major cities in India.

What is the quality of the assembly?

We aim for a high-quality assembly with N50 > 100 kb and minimal contigs, suitable for detailed genomic analysis.

Who will interpret the results?

The results are interpreted by our team of geneticists and infectious disease specialists, and a detailed report is provided.

Are there any risks associated with this test?

No physical risks are involved as the test is performed on extracted DNA.


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Editorial accountability

Written by: Lookesh Sharma, Medical Content Writer
Reviewed by: Dr SHAILAJA RAGHUNATH MURDESHWAR , Consultant Physician
Last updated: 07 Sep 2026

DNA Labs India

Address: 2nd Floor, Prashanthi Arcade, 202, Amberpet Main Rd, Amberpet, Hyderabad - 500013, Telangana, India

Phone:+919395142800

Enquiries are answered by our laboratory team. No payment is collected on this website.

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