Overview
Bacterial V3-V4 16S rRNA amplicon sequencing is a cutting-edge molecular diagnostic technique used to identify bacterial species present in a clinical sample. The 16S rRNA gene is highly conserved across bacteria, but its V3-V4 hypervariable regions contain species-specific sequences. By amplifying and sequencing these regions, the test can precisely identify bacteria, including those that are difficult or impossible to culture using conventional methods. This test is particularly valuable in cases of culture-negative infections, polymicrobial infections, or when a rapid and accurate etiological diagnosis is critical. DNA Labs India offers this advanced test at an affordable cost of INR 7000, making it accessible to patients across the country. The test is performed on extracted DNA from various sample types, including blood, urine, stool, body fluids, or tissue. With a turnaround time of 4 weeks, the test provides comprehensive results that aid clinicians in making informed treatment decisions. The test is performed using next-generation sequencing (NGS) technology, ensuring high sensitivity and specificity. It is an essential tool for infectious disease specialists, microbiologists, and clinicians dealing with complex bacterial infections.
To be completed — reclassified from Tests on 2026-11-13.
The primary purpose of Bacterial V3-V4 16S rRNA amplicon sequencing is to identify the causative bacterial agent in patients with suspected bacterial infections, especially when conventional cultures fail. It helps in detecting uncommon, fastidious, or slow-growing bacteria, and provides a definitive diagnosis for targeted antimicrobial therapy. The test is also useful in epidemiological studies and outbreak investigations.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: Reports are typically delivered within 4 weeks after sample collection.
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | Reports are typically delivered within 4... | ₹7,000 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is Bacterial V3-V4 16S rRNA Amplicon Sequencing?
It is a molecular diagnostic test that identifies bacterial species by sequencing the V3-V4 region of the 16S rRNA gene.
Why is this test done?
It is done to identify bacteria causing infections, especially when conventional cultures are negative or difficult.
What sample is required?
Extracted DNA from blood, urine, stool, body fluid, or tissue.
Do I need to fast before the test?
No, fasting is not required.
How long does it take to get results?
Results are available in 4 weeks.
What is the cost of the test?
The cost is INR 7000.
Is home sample collection available?
Yes, free home sample collection is available for online bookings.
Can this test detect all bacteria?
It can detect most bacteria, but some may not be identified due to database limitations.
Is the test covered by insurance?
Insurance coverage is not guaranteed; please check with your provider.
What are the symptoms of a bacterial infection?
Common symptoms include fever, chills, fatigue, body aches, nausea, and diarrhea.
How is the test performed?
DNA is extracted from the sample, amplified by PCR, and sequenced using NGS technology.
Are there any risks?
Risks are minimal, mainly related to sample collection like bruising.
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