Overview
ddRAD (double-digest restriction site-associated DNA) library preparation is a cutting-edge technique used for genome-wide genotyping and single nucleotide polymorphism (SNP) discovery. It involves the digestion of genomic DNA with two restriction enzymes, followed by ligation of adapters and PCR amplification to create a sequencing library. This method is widely used in population genetics, conservation biology, and genome-wide association studies (GWAS) due to its cost-effectiveness and high resolution. At DNA Labs India, we offer a comprehensive ddRAD library preparation service at an affordable price of INR 145000. Our state-of-the-art facility ensures high-quality library construction with rigorous quality control checks at every step. We accept extracted DNA samples and provide results within one week. Our experienced team of geneticists and molecular biologists ensures that your project receives personalized attention and reliable data. Whether you are a researcher, academic institution, or biotech company, DNA Labs India is your trusted partner for genomic services.
To be completed — reclassified from Tests on 2026-11-13.
The primary purpose of ddRAD library preparation is to generate a reduced-representation sequencing library that targets a subset of the genome, enabling cost-effective genotyping of many individuals. It is used for SNP discovery, population structure analysis, linkage mapping, and phylogenetic studies. By focusing on specific restriction sites, ddRAD reduces genome complexity, making it ideal for non-model organisms and large-scale studies.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: Reports are delivered within 1 week after sample submission.
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | Reports are delivered within 1 week afte... | ₹145,000 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is ddRAD library preparation?
ddRAD (double-digest restriction site-associated DNA) library preparation is a method used to create a reduced-representation sequencing library by digesting DNA with two restriction enzymes, ligating adapters, and amplifying the fragments. It is used for genotyping and SNP discovery.
What is the cost of ddRAD library preparation at DNA Labs India?
The cost is INR 145000, which includes DNA quality check, library preparation, QC, and basic bioinformatics analysis.
What sample type is required for ddRAD library preparation?
Extracted DNA is required. The DNA should be of high quality (A260/A280 1.8-2.0) and quantity (? 50 ng/µL).
How long does it take to get results?
The turnaround time is 1 week after sample submission.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings across India.
What is the difference between ddRAD and RAD-seq?
ddRAD uses two restriction enzymes, which provides more consistent fragment sizes and higher efficiency compared to RAD-seq, which uses one enzyme.
Can I use ddRAD for non-model organisms?
Yes, ddRAD is particularly useful for non-model organisms because it does not require a reference genome.
What quality checks are performed?
We perform DNA quality and quantity checks, digestion efficiency, ligation efficiency, and library concentration and fragment size analysis.
Is the test covered by insurance?
No, this test is not typically covered by insurance. However, you can check with your provider.
What is the recommended DNA amount?
We recommend 1-2 µg of DNA with a concentration of at least 50 ng/µL.
Do you provide bioinformatics support?
Yes, basic bioinformatics analysis is included in the price. Additional analysis can be requested at an extra cost.
How should I ship my DNA sample?
Ship the sample in a sterile tube on dry ice or cold packs to maintain stability. Ensure proper labeling.
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