Overview
Eukaryotic mRNA sequencing (mRNA-seq) is a powerful technique that profiles the transcriptome of eukaryotic cells by sequencing messenger RNA molecules. De novo data analysis refers to the assembly of transcript sequences without relying on a reference genome. This approach is essential for organisms with incomplete or absent reference genomes, and for discovering novel transcripts, splice variants, and fusion genes that may be implicated in diseases such as cancer and genetic disorders. At DNA Labs India, we provide comprehensive de novo mRNA sequencing analysis at an affordable cost of INR 24000. Our state-of-the-art bioinformatics pipeline ensures accurate assembly, quantification, and annotation of transcripts. This service is ideal for researchers and clinicians seeking to identify disease-associated genetic alterations, especially in cases where standard reference-based analysis may miss critical variants. With a turnaround time of 4 weeks and free home sample collection across major Indian cities, DNA Labs India delivers reliable results with expert interpretation. Our team of geneticists and bioinformaticians works closely with referring physicians to tailor the analysis to specific clinical or research questions, ensuring actionable insights for patient care or research advancement.
To be completed — reclassified from Tests on 2026-11-13.
The purpose of eukaryotic mRNA sequencing de novo data analysis is to determine the complete transcriptome sequence of a eukaryotic organism without a reference genome. This allows for the identification of novel transcripts, alternative splicing events, and gene fusions that may be associated with diseases. It is particularly useful in oncology for detecting fusion genes in tumors, in rare genetic disease diagnosis for identifying pathogenic splice variants, and in research for characterizing transcriptomes of non-model organisms. The analysis provides a comprehensive view of gene expression and isoform diversity, enabling a deeper understanding of disease mechanisms and potential therapeutic targets.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: Reports are typically delivered within 4 weeks from sample receipt.
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | Reports are typically delivered within 4... | ₹24,000 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is eukaryotic mRNA sequencing de novo data analysis?
It is a technique to sequence mRNA from eukaryotic cells and assemble the transcriptome without a reference genome, allowing discovery of novel transcripts and splice variants.
What is the cost of this test at DNA Labs India?
The cost is INR 24000, which includes bioinformatics analysis and report interpretation.
What sample type is required?
Extracted DNA is the sample type, but typically RNA is extracted from blood or tissue samples. The lab will guide you on the specific requirements.
How long does it take to get results?
The turnaround time is 4 weeks from sample receipt.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings across major cities in India.
What is the difference between de novo and reference-based analysis?
De novo analysis assembles transcripts without a reference genome, while reference-based analysis aligns reads to a known genome. De novo is useful for non-model organisms and novel transcript discovery.
Can this test detect cancer-related fusion genes?
Yes, de novo mRNA sequencing can identify fusion transcripts that may be associated with cancer.
Is fasting required before the test?
No, fasting is not required for this test.
What is the sample volume needed?
Typically 1-2 µg of extracted RNA is required, but the lab will provide specific instructions.
Are there any risks associated with the test?
The risks are minimal, mainly related to blood draw or biopsy procedures. Your physician will explain them.
How should the sample be stored before shipping?
Blood samples should be stored in PAXgene tubes at room temperature for up to 3 days. Tissue samples should be placed in RNA stabilization solution and stored at 4°C.
Will my insurance cover this test?
Insurance coverage varies. We recommend checking with your insurance provider. We also offer cashless options for some schemes.
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