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Sequencing service

Fungal ITS2 Amplicon Sequencing and Analysis

Reviewed for accuracy
₹10,000
Per sample
final quote on confirmation
Results are typically available wi...
Turnaround
from sample receipt
Extracted DNA
Sample
input material
Enquiry
How to order
no payment online

Overview

Fungal ITS2 amplicon sequencing is a molecular diagnostic technique that targets the Internal Transcribed Spacer 2 (ITS2) region of the fungal ribosomal DNA. This region is highly variable and serves as a universal barcode for fungal species identification. The test involves PCR amplification of the ITS2 region from extracted DNA, followed by next-generation sequencing (NGS) and bioinformatics analysis against reference databases. This method provides high-resolution identification of fungi, including species that are difficult to culture or are slow-growing. It is particularly useful in cases of invasive fungal infections, where rapid and accurate identification is critical for patient management. At DNA Labs India, we offer this advanced test at an affordable cost of INR 10000, with free home sample collection across major cities. The test is performed by experienced molecular biologists and microbiologists, ensuring reliable results. The turnaround time is 8 weeks, which includes sequencing and comprehensive analysis. This test is not intended for routine screening but is reserved for cases where fungal infection is suspected and conventional methods have failed to provide a definitive diagnosis.

To be completed — reclassified from Tests on 2026-11-13.

The purpose of Fungal ITS2 Amplicon Sequencing is to identify the exact fungal species present in a clinical sample. This is essential for guiding antifungal therapy, as different species have varying susceptibility to antifungal agents. It also helps in epidemiological studies and outbreak investigations. The test is particularly valuable for immunocompromised patients, where fungal infections can be life-threatening and require prompt, targeted treatment.

Sample requirements

What to send us

Sample type: Extracted DNA
Volume: 10-50 ng/µL (as per lab instruction)
Container: Sterile microcentrifuge tube
Collection: Blood or tissue sample as per clinical indication

Deliverables & turnaround

What you receive

Turnaround: Results are typically available within 8 weeks after the sample is received by the laboratory.

Pricing

Transparent pricing

OptionTurnaroundPrice (INR)Unit
StandardResults are typically available within 8...₹10,000per sample

Limitations & precautions

What this service cannot do

Test does not differentiate between viable and non-viable organisms
May not detect all fungal species due to incomplete reference databases
Requires sufficient DNA quantity and quality
Results should be correlated with clinical findings and other diagnostic tests
Not a substitute for culture and susceptibility testing

Questions customers ask

Frequently asked questions

What is Fungal ITS2 Amplicon Sequencing?

It is a molecular technique that sequences the ITS2 region of fungal DNA to identify the specific fungus in a sample.

Why is ITS2 region used for fungal identification?

The ITS2 region is highly variable among fungal species, making it an ideal barcode for species-level identification.

What sample is required for this test?

Extracted DNA is required. The sample can be from blood, tissue, or other clinical specimens, depending on the infection site.

How long does the test take?

The turnaround time is 8 weeks, which includes sequencing and analysis.

Is fasting required before the test?

No, fasting is not required for this test.

Can this test detect all types of fungi?

It can detect most clinically relevant fungi, but some rare species may not be identified due to incomplete databases.

Is home sample collection available?

Yes, we offer free home sample collection for online bookings across many cities in India.

What is the cost of the test?

The test costs INR 10000, which is a discounted price.

How should I interpret a positive result?

A positive result indicates the presence of fungal DNA, and the species is identified. Your doctor will correlate this with your symptoms.

Are there any risks associated with the test?

The test is safe. The only risk is minor bleeding or bruising at the blood draw site.

Can I take antifungal medications before the test?

Yes, but inform your doctor, as it may affect the test results.

How do I book this test?

You can book online through our website or call our customer care number. Home collection is available.


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Editorial accountability

Written by: Nitin Yadav, Medical Content Writer
Reviewed by: Dr Kothari Ramesh Shantilal , Consultant Microbiologist
Last updated: 07 Sep 2026

DNA Labs India

Address: 2nd Floor, Prashanthi Arcade, 202, Amberpet Main Rd, Amberpet, Hyderabad - 500013, Telangana, India

Phone:+919395142800

Enquiries are answered by our laboratory team. No payment is collected on this website.

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