Overview
Polyploid plants, which carry multiple sets of chromosomes, present unique challenges in genome assembly due to high sequence similarity among homeologous chromosomes. Traditional reference-based methods often fail, making de novo assembly essential. This service utilizes Illumina sequencing to generate high-depth short reads, which are then assembled into contigs and scaffolds using specialized algorithms that handle polyploid complexity. The assembled genome is subsequently annotated to identify genes, regulatory elements, non-coding RNAs, and repetitive sequences. This comprehensive approach enables researchers to explore the genetic basis of traits such as vigor, stress tolerance, and disease resistance. At DNA Labs India, we provide end-to-end solutions from DNA extraction to final annotated genome, ensuring high accuracy and reliability. Our bioinformatics team employs state-of-the-art tools and quality checks to deliver a genome assembly that meets international standards. The service is ideal for plant breeding programs, evolutionary studies, and functional genomics research. With a turnaround time of 8 weeks, we offer detailed reports including assembly statistics, annotation summaries, and comparative analyses. The cost of INR 560,000 covers all steps, including library preparation, sequencing, and computational analysis. We also offer free home sample collection across major cities in India for your convenience.
To be completed — reclassified from Tests on 2026-11-13.
The primary purpose of this test is to generate a high-quality reference genome for polyploid plant species, enabling detailed genetic and genomic studies. It facilitates the identification of genes responsible for agronomically important traits, supports marker-assisted breeding, and aids in understanding genome evolution. The annotated genome serves as a foundation for functional genomics, comparative genomics, and genetic improvement programs.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: Reports are delivered within 8 weeks from the date of sample receipt.
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | Reports are delivered within 8 weeks fro... | ₹560,000 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is polyploid plant genome de novo assembly?
It is the process of constructing a reference genome for a polyploid plant species from scratch using sequencing data, without a reference genome.
Why is Illumina sequencing used for this test?
Illumina offers high accuracy and high throughput, making it suitable for generating deep coverage required for polyploid genome assembly.
What sample is required?
Extracted DNA of high molecular weight, typically 5-10 µg, from the plant tissue.
How long does the test take?
The turnaround time is 8 weeks from sample receipt.
What is included in the cost?
The cost includes library preparation, sequencing, bioinformatics analysis, assembly, annotation, and report generation.
Can you handle any polyploid plant species?
Yes, we can process various polyploid species, but genome complexity may affect assembly quality.
What quality metrics will be reported?
We report assembly size, N50, BUSCO completeness, GC content, and gene annotation statistics.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings across major cities in India.
How should the DNA sample be shipped?
Ship on dry ice in a DNA-free tube with proper labeling and a completed requisition form.
Will the data be kept confidential?
Yes, all data is handled with strict confidentiality and security protocols.
Can this service be used for clinical diagnosis?
No, this is a research-use-only service and not intended for clinical diagnostics.
What if the assembly quality is poor?
We may recommend additional sequencing or alternative approaches, but additional costs may apply.
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