Short Description

DNA QC and quantitation using Nanodrop, preparation of 96 ddRAD libraries using MseI/ EcoRI restriction enzymes, multiplexing of barcoded libraries and 2x150 PE Illumina Hiseq sequencing to generate total of 400 million reads (R1+R2) per library pool. Variant calling and genotyping. Data will be delivered as FASTQ sequences as well as genotype information in spreadsheet.

PreTest Information

Detail Description

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ddRAD Sequencing and Primary Analysis-96 Samples Cost INR:480000 - DNA Labs India

ddRAD (double digest restriction-site associated DNA) sequencing is a cost-effective and reliable method to analyze genetic variation at a genome-wide level. It involves the digestion of genomic DNA with two restriction enzymes, followed by adapter ligation and PCR amplification to create a library of fragments. These fragments are then sequenced using high-throughput sequencing platforms.

At DNA Labs India, we offer ddRAD sequencing and primary analysis for 96 samples at a cost of INR 480000. This service can help in accurate diagnosis of various symptoms and genetic conditions.

Process

The process of ddRAD sequencing and primary analysis involves the following steps:

1. Genomic DNA extraction
2. Double digestion of genomic DNA with two restriction enzymes
3. Adapter ligation and PCR amplification
4. Library preparation and sequencing using Illumina HiSeq platform
5. Primary analysis of raw sequencing data to identify genetic variants and mutations

The primary analysis includes quality control, read mapping, variant calling, and annotation. The results are then interpreted and reported to the client for further analysis and validation.

Cost

The cost of ddRAD sequencing and primary analysis for 96 samples is INR 480000. This includes all the steps mentioned above, as well as data analysis and reporting.

Symptoms Diagnosis

ddRAD sequencing and primary analysis can help in accurate diagnosis of various symptoms and genetic conditions, such as:

• Hereditary cancer syndromes
• Neurological disorders
• Cardiovascular diseases
• Metabolic disorders
• Autoimmune diseases
• And many more

By identifying the genetic variants and mutations associated with these conditions, we can provide valuable insights into disease pathogenesis, risk assessment, and personalized treatment options.

Conclusion

ddRAD sequencing and primary analysis is a powerful tool for genetic analysis and diagnosis. At DNA Labs India, we offer this service at a cost-effective price, without compromising on quality and accuracy. Contact us today to learn more about how we can help you with your genetic analysis needs.