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Immunophenotyping by Flow Cytometry CD4 Test

DNA Labs India | ISO 9001:2015 Certified

Immunophenotyping by Flow Cytometry CD4 Test

Short Name: CD4 Test

Also known as: CD4 Count Test, CD4 Enumeration, T-cell Subset Analysis

Immunophenotyping by Flow Cytometry CD4 Test test available at DNA Labs India for ₹1,872. Uses Flow Cytometry on Whole Blood or Bone Marrow samples. Results in Same day report delivery. Free home collection in 300+ cities across India.

Flow Cytometry🏠 Home Collection

✍️ Written By

Overview

To quantify CD4 T-cell counts for monitoring immune function in HIV/AIDS and other immunological conditions, aiding in diagnosis and treatment planning.

Test Code
1077
Price
₹1,872
Sample Type
Whole Blood or Bone Marrow
Result Time
Same day report delivery
Fasting Required
No
Method
Flow Cytometry
Step 1

Sample Collection

Provide brief clinical history, including any relevant medical conditions, medications, and reason for testing.

Method: Venipuncture or Bone Marrow Aspiration

Step 2

Laboratory Analysis

A blood sample will be drawn from a vein in your arm. For bone marrow aspiration, a needle will be inserted into the bone.

Step 3

Report Delivery

Apply pressure to the puncture site to prevent bleeding. Resume normal activities, avoiding strenuous exercise for a few hours.

Timeline: Same day report delivery

Patient Instructions

1
Before the Test:Provide brief clinical history and any relevant medical details. No specific preparation is required, but inform the healthcare provider about any medications.
2
During the Test:A blood sample will be collected via venipuncture. The process is quick and relatively painless, taking only a few minutes.
3
After the Test:Apply pressure to the puncture site to prevent bleeding. You can resume normal activities immediately. Results will be available on the same day.

About This Test

Who Should Get This Test

To quantify CD4 T-cell counts for monitoring immune function in HIV/AIDS and other immunological conditions, aiding in diagnosis and treatment planning.

How to Prepare

  • Ship immediately at 18-22°C.
  • Do not refrigerate or freeze the sample.
  • Specify time, date, and clinical details on the test request form.

Doctor's Notes

"This test is crucial for monitoring CD4 counts in HIV patients and assessing immune reconstitution post-transplant, guiding treatment decisions effectively."

Test Parameters & Specifications

Sample TypeWhole Blood or Bone Marrow
Sample Volume3 mL whole blood or 2 mL bone marrow
ContainerEDTA tube and Sodium Heparin tube
Collection MethodVenipuncture or Bone Marrow Aspiration

Sample Stability

Room Temperature: 24 hours
Refrigerator: Not Applicable
Frozen: Not Applicable
Sample Rejection Criteria:
  • Hemolyzed or clotted samples
  • Improperly labeled or insufficient volume tubes
  • Delayed transport beyond 24 hours at room temperature

Understanding Your Results

Interpretation of CD4 count results should be done by a healthcare provider, considering the patient's overall clinical context, medical history, and other test results.
Low CD4 count (<200 cells/μL) indicates severe immunosuppression, commonly seen in advanced HIV/AIDS.
Normal CD4 count (500-1500 cells/μL) suggests adequate immune function.
Trends over time are more important than single values for monitoring.
CD4 percentage provides additional context and may be more stable than absolute counts.
Abnormal results may require further testing or adjustment in therapy.
⚠️ When to Consult a Doctor:

If CD4 count is below normal range, or if symptoms of immune deficiency such as frequent infections, fatigue, or weight loss persist.

Limitations

  • ⚠Requires fresh sample for optimal accuracy
  • ⚠Results may vary with laboratory techniques
  • ⚠Not diagnostic alone for specific diseases; must be correlated clinically

Risks & Considerations

  • ●Minor bruising or pain at the blood draw site
  • ●Rare risk of infection or hematoma
  • ●For bone marrow aspiration, additional risks include pain, bleeding, and infection

Interfering Factors

  • ●Sample mishandling or delayed transport
  • ●Time of collection (diurnal variations)
  • ●Recent infections or vaccinations
  • ●Medications affecting immune system

Frequently Asked Questions

What is the Immunophenotyping by Flow Cytometry CD4 Test?
This test measures the number of CD4 T lymphocytes in your blood using flow cytometry to assess immune function, especially in HIV/AIDS and other immunological conditions.
Why is the CD4 test important for HIV patients?
It helps monitor the progression of HIV by tracking CD4 cell counts, which decline as HIV weakens the immune system, guiding treatment decisions.
How is the CD4 test performed?
A blood sample is drawn from your arm and analyzed in a laboratory using flow cytometry to identify and count CD4 cells based on surface markers.
What is the cost of the CD4 test in India?
The cost at DNA Labs India is INR 1872, which includes free home sample collection and same-day report delivery.
How long does it take to get CD4 test results?
Results are typically available on the same day if the sample is collected before 9 am, with reports delivered via online portal, email, or WhatsApp.
What are normal CD4 count values?
Normal CD4 counts for adults range from 500 to 1500 cells per microliter of blood, but values can vary based on age, sex, and health status.
Can the CD4 test diagnose HIV?
No, the CD4 test does not diagnose HIV; it monitors immune function in known HIV-positive patients. HIV diagnosis requires specific antibody or antigen tests.
How often should CD4 tests be done?
For HIV patients, CD4 tests are recommended every 3-6 months or as advised by a healthcare provider to monitor immune health.
What do low CD4 counts indicate?
Low CD4 counts indicate a weakened immune system, which can be due to HIV/AIDS, other infections, or immunodeficiency disorders, increasing infection risk.
Is fasting required for the CD4 test?
No, fasting is not required for this test. However, providing accurate clinical history is recommended for proper interpretation.
What are the risks of the CD4 test?
Risks are minimal and may include minor bruising or pain at the blood draw site. Serious complications are rare.
How can I prepare for the CD4 test?
No special preparation is needed. Ensure to provide your clinical history and follow any instructions from your healthcare provider for sample collection.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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