Paroxysmal Nocturnal Hemoglobinuria (PNH) Confirmatory Test
Short Name: PNH Confirmatory Test
Also known as: PNH Flow Cytometry, PNH Clone Analysis, GPI-anchored protein deficiency test
Paroxysmal Nocturnal Hemoglobinuria (PNH) Confirmatory Test test available at DNA Labs India for ₹8,500. Uses Flow Cytometry on Whole blood samples. Results in Same-day report if the sample reaches the laboratory by 9 am.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
This test is primarily used to evaluate the percentage of granulocytes and monocytes in blood that express CD55, CD14, CD24, CD59 and FLAER, and those which do not express them. These antigens are absent on the hematopoietic cells of patients suffering from PNH.
- Test Code
- 3527
- Price
- ₹8,500
- Sample Type
- Whole blood
- Result Time
- Same-day report if the sample reaches the laboratory by 9 am.
- Fasting Required
- No
- Method
- Flow Cytometry
Sample Collection
No fasting is required. Please provide a brief clinical history and inform the laboratory about any recent blood transfusions.
Method: Peripheral venous blood draw
Laboratory Analysis
Two blood samples will be collected: one in a Lavender Top EDTA tube and one in a Green Top Sodium Heparin tube. The procedure takes about 5-10 minutes.
Report Delivery
You may resume normal diet and activities. The samples must be transported immediately to the laboratory at room temperature. Do not refrigerate or freeze.
Timeline: Same-day report if the sample reaches the laboratory by 9 am.
Patient Instructions
About This Test
Who Should Get This Test
This test is primarily used to evaluate the percentage of granulocytes and monocytes in blood that express CD55, CD14, CD24, CD59 and FLAER, and those which do not express them. These antigens are absent on the hematopoietic cells of patients suffering from PNH.
How to Prepare
- Specify time, date, and clinical details on the test request form
- Transport immediately at 18-22°C
- DO NOT REFRIGERATE OR FREEZE
- Ensure both EDTA and Sodium Heparin tubes are filled to the required volume
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"Flow cytometry is the gold-standard confirmatory test for PNH. Detection of a GPI-anchored protein-deficient clone should always be interpreted along with clinical findings, CBC, reticulocyte count, LDH, and haptoglobin."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Clotted sample
- Hemolyzed sample
- Refrigerated or frozen sample
- Sample received after 24 hours of collection
- Insufficient sample volume
Understanding Your Results
Normal expression of CD55, CD59, FLAER on granulocytes/monocytes and CD59 on RBC
No PNH clone detected; PNH is unlikely.
Partial deficiency of GPI-anchored proteins
Type II PNH clone may be present; clinical correlation advised.
Complete absence of GPI-anchored proteins on a cell population
Type III PNH clone present; consistent with PNH.
Consult a hematologist if you experience dark urine, unexplained weakness, recurrent blood clots, or unexplained anemia. If PNH is confirmed, regular follow-up is necessary to monitor clone size and prevent complications.
Limitations
- ⚠Low-level PNH clones may require high-sensitivity flow cytometry protocols
- ⚠Flow cytometry results must be correlated with clinical and hematological findings
- ⚠Recent transfusion can affect assessment of CD59 on red blood cells
- ⚠Bone marrow transplantation can change the cell populations being measured
Risks & Considerations
- ●Minor pain or bruising at the needle site
- ●Dizziness or lightheadedness
- ●Very low risk of infection
Interfering Factors
- ●Recent blood transfusion may dilute the PNH clone and cause false-negative results
- ●Clotted or hemolyzed blood sample
- ●Delayed processing beyond 24 hours
- ●Transport at refrigerated or frozen temperature
- ●Recent hematopoietic stem cell transplant may alter results
Compare With Similar Tests
| Test | Paroxysmal Nocturnal Hemoglobinuria (PNH) Confirmatory Test Test | |||
|---|---|---|---|---|
| Comparison | Paroxysmal Nocturnal Hemoglobinuria (PNH) Confirmatory Test Test |
Frequently Asked Questions
What is PNH?
Why is a confirmatory test needed for PNH?
How is the PNH confirmatory test performed?
Does the PNH confirmatory test require fasting?
What does flow cytometry measure in this test?
What is FLAER?
How much does the PNH confirmatory test cost at DNA Labs India?
When will I get the report?
What sample is required for this test?
Are there any risks in this test?
Can a recent blood transfusion affect the test result?
Who should get tested for PNH?
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