Overview
12S rRNA sequencing is a specialized DNA sequencing test that focuses on the mitochondrial genome, specifically the 12S ribosomal RNA gene. This gene plays a vital role in protein synthesis within mitochondria, the energy-producing organelles in cells. By sequencing this region, scientists can identify mutations or variations that may indicate mitochondrial disorders, which are genetic conditions affecting energy production and can lead to a wide range of symptoms. The test is particularly useful for diagnosing disorders caused by mitochondrial DNA mutations, providing insights into conditions that impact multiple organ systems. It is a relatively affordable and accessible option in India, with a cost of INR 5600, and is often recommended when patients present with unexplained symptoms such as fatigue, muscle weakness, or neurological issues. The process involves extracting DNA from a sample, amplifying the 12S rRNA gene using PCR, and sequencing it to detect any abnormalities. Results from this test can guide further diagnostic steps and treatment plans, making it a valuable tool in genetic medicine.
To be completed — reclassified from Tests on 2026-11-13.
The purpose of 12S rRNA sequencing is to detect mutations in the 12S ribosomal RNA gene of the mitochondrial genome, which can help diagnose mitochondrial disorders. These disorders often present with diverse symptoms, and this test provides a targeted approach to identify genetic causes, aiding in clinical management and family counseling.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: 2 weeks
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | 2 weeks | ₹5,600 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is 12S rRNA sequencing?
12S rRNA sequencing is a DNA sequencing test that analyzes the 12S ribosomal RNA gene in the mitochondrial genome to detect mutations associated with mitochondrial disorders.
How much does 12S rRNA sequencing cost in India?
The cost of 12S rRNA sequencing in India is INR 5600, which includes home sample collection across major cities.
What are the symptoms of mitochondrial disorders?
Common symptoms include fatigue, muscle weakness, hearing or vision loss, seizures, developmental delays, heart problems, and gastrointestinal issues.
How is the test performed?
The test involves collecting a DNA sample, extracting DNA, amplifying the 12S rRNA gene using PCR, and sequencing it to identify mutations.
Is fasting required for this test?
No, fasting is not required for 12S rRNA sequencing.
How long does it take to get results?
Results are typically available within 2 weeks after sample collection.
Is the test covered by insurance?
Insurance coverage may vary; it is advisable to check with your insurance provider before undergoing the test.
What sample is needed for the test?
The test requires extracted DNA, usually obtained from a blood or tissue sample.
Are there any risks associated with the test?
The test has minimal risks, primarily related to sample collection, such as slight discomfort or bruising.
How accurate is 12S rRNA sequencing?
The test is highly accurate for detecting mutations in the 12S rRNA gene, but it may not identify all mitochondrial disorders.
Can this test detect all mitochondrial disorders?
No, it focuses on the 12S rRNA gene; other mitochondrial disorders may require additional tests like whole mitochondrial genome sequencing.
What should I do if the test results are abnormal?
If results are abnormal, consult a geneticist or specialist for further evaluation, confirmation testing, and management options.
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