Overview
Bacterial transcriptome sequencing is a cutting-edge molecular technique that profiles the complete set of RNA transcripts produced by a bacterium under specific conditions. Unlike whole-genome sequencing, which reveals the genetic potential, transcriptome sequencing shows which genes are actively expressed, offering a dynamic snapshot of bacterial physiology. This is crucial for understanding pathogenicity, host interactions, and antibiotic resistance mechanisms. De novo analysis is employed when a reference genome is unavailable or incomplete, allowing the assembly of transcripts from scratch. This approach is particularly valuable for novel or poorly characterized bacterial strains. At DNA Labs India, the test involves isolation of bacterial RNA, conversion to cDNA, sequencing on high-throughput platforms, and subsequent bioinformatics analysis to identify expressed genes, splice variants, and novel transcripts. The resulting data aids clinicians in diagnosing infections, predicting virulence, and tailoring antimicrobial therapy. The test is performed on extracted DNA samples, ensuring compatibility with various clinical specimens. With a turnaround time of 8 weeks, the comprehensive report provides actionable insights for infectious disease management. The cost of INR 30,000 includes all steps from RNA isolation to final annotation, making it an affordable option for advanced bacterial analysis in India.
To be completed — reclassified from Tests on 2026-11-13.
The primary purpose of bacterial transcriptome sequencing and de novo analysis is to identify and quantify the genes actively expressed by a bacterial pathogen. This information is critical for: 1) Understanding the molecular basis of bacterial virulence, 2) Detecting antibiotic resistance gene expression, 3) Identifying novel genes that may serve as therapeutic targets, 4) Characterizing bacterial strains with no reference genome, and 5) Supporting clinical decisions in complex or recurrent infections. By analyzing the transcriptome, clinicians can gain insights into the real-time metabolic and pathogenic state of the bacteria, which is not possible with conventional culture or PCR methods. This test is especially useful when standard diagnostic approaches fail to identify the causative organism or when there is a need to understand the mechanisms of drug resistance.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: Reports are typically delivered within 8 weeks from sample receipt.
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | Reports are typically delivered within 8... | ₹30,000 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is bacterial transcriptome sequencing?
Bacterial transcriptome sequencing is a technique that profiles all RNA transcripts produced by a bacterium at a given time, revealing which genes are actively expressed. This helps understand bacterial behavior, virulence, and antibiotic resistance.
What is de novo analysis in this context?
De novo analysis refers to assembling and analyzing transcriptome data without a reference genome. It is useful for novel or poorly characterized bacteria, allowing identification of new genes and transcripts.
What sample is required for this test?
The test requires extracted DNA from the bacterial sample. The sample type is specified as 'Extracted DNA', which may be derived from blood, tissue, or other clinical specimens.
How long does it take to get results?
The turnaround time is 8 weeks from the date of sample receipt. Reports are delivered via online portal, email, or WhatsApp.
Is fasting required before the test?
No, fasting is not required for this test. You can eat and drink normally before sample collection.
What is the cost of the test?
The cost is INR 30,000, which includes all steps from RNA isolation to final annotation. Home sample collection is available at no extra charge.
Can this test detect antibiotic resistance?
Yes, the test can identify expression of antibiotic resistance genes, providing insights into potential resistance mechanisms that may guide treatment decisions.
Is home sample collection available?
Yes, DNA Labs India offers free home sample collection for online bookings across major cities in India.
What are the symptoms of bacterial infections that may warrant this test?
Common symptoms include fever, pain, swelling, redness, difficulty breathing, nausea, vomiting, diarrhea, and joint pain. If you have persistent or severe symptoms, consult your doctor.
How is the test performed?
The test involves isolation of bacterial RNA, conversion to cDNA, sequencing using next-generation technology, de novo assembly, and annotation of transcripts to identify genes and variants.
Are there any risks associated with the test?
The test is minimally invasive. Risks are limited to those of routine blood collection, such as slight pain or bruising at the puncture site.
Who should consider this test?
This test is recommended for patients with recurrent or resistant bacterial infections, suspected novel pathogens, or when standard diagnostics are inconclusive. It is also useful for research purposes.
Continue here
Related services
Enquiry
Request a quote or ask a question
Editorial accountability