Overview
ddRAD sequencing, or double digest restriction-site associated DNA sequencing, is a powerful molecular technique used for the identification and analysis of genetic variations across organisms. This method involves digesting DNA with two restriction enzymes, such as EcoRI and MspI, followed by adapter ligation and sequencing, focusing on specific genomic regions to provide a reduced representation of the genome. It is widely employed in population genetics, phylogenetics, comparative genomics, and disease association studies. For instance, ddRAD sequencing can uncover genetic variants linked to conditions such as cancer, diabetes, and cardiovascular diseases, aiding in diagnosis and personalized treatment. Additionally, it is instrumental in plant and animal breeding programs by identifying markers for desirable traits like yield, disease resistance, and stress tolerance. The cost for ddRAD sequencing of 96 samples in India is INR 395,000, which includes library preparation, sequencing, and data analysis, making it a cost-effective alternative to whole-genome sequencing. At DNA Labs India, we offer this service with free home sample collection across numerous cities, ensuring accessibility and convenience for researchers and clinicians. Our state-of-the-art sequencing platforms ensure high accuracy and reproducibility, with results delivered within 5 weeks. This test is suitable for both research and clinical applications, providing valuable insights into genetic diversity and disease mechanisms.
To be completed — reclassified from Tests on 2026-11-13.
ddRAD sequencing is used for high-throughput analysis of genetic variations, enabling applications in disease diagnosis, population genetics, phylogenetics, and breeding programs to identify traits and improve outcomes.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: 5 weeks
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | 5 weeks | ₹395,000 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is ddRAD sequencing?
ddRAD sequencing is a reduced representation sequencing technique that uses double digest restriction enzymes to target specific genomic regions for genetic variation analysis.
How much does ddRAD sequencing for 96 samples cost?
The cost for ddRAD sequencing of 96 samples in India is INR 395,000, which includes library preparation, sequencing, and data analysis.
What are the applications of ddRAD sequencing?
It is used in population genetics, phylogenetics, disease diagnosis, comparative genomics, and plant/animal breeding programs.
How is ddRAD sequencing different from whole-genome sequencing?
ddRAD sequencing focuses on specific genomic regions, making it more cost-effective and faster, while whole-genome sequencing covers the entire genome but is more expensive.
What sample type is required for ddRAD sequencing?
Extracted DNA is required, and it should be of high quality and quantity for optimal results.
How long does it take to get results?
The turnaround time for ddRAD sequencing is approximately 5 weeks from sample receipt.
Is home sample collection available?
Yes, DNA Labs India offers free home sample collection for online bookings across many cities in India.
What does the cost include?
The cost includes library preparation, sequencing, and data analysis for 96 samples.
Can ddRAD sequencing be used for disease diagnosis?
Yes, it can identify genetic variations associated with diseases like cancer and diabetes, aiding in diagnosis and personalized medicine.
What are the limitations of ddRAD sequencing?
Limitations include not being suitable for whole-genome analysis, requiring high-quality DNA, and potentially missing some genetic variations.
How should I prepare my samples?
Ensure DNA is extracted and purified, stored at -20°C, and labeled correctly to avoid degradation or contamination.
Is genetic counseling recommended after testing?
Yes, especially if pathogenic variants are found, genetic counseling can help interpret results and guide clinical decisions.
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