High Resolution HLA Typing Test Price & Report Details | भारत में एचएलए टाइपिंग टेस्ट की कीमत
High‑resolution, next‑generation sequencing (NGS) based HLA typing for transplant compatibility, disease association, and pharmacogenetics
| Parameter | Details |
|---|---|
| Price (Single Person) | ₹8,000 (all‑inclusive, free home collection) – fixed for individual, not multi‑person package |
| Methodology | Illumina Next‑Generation Sequencing (NGS) with protein‑level ARS |
| Sample Type | Whole blood (5 mL) in EDTA tube |
| Turnaround Time | 10–12 working days |
| Report Format | Digital PDF with G‑Codes, NMDP codes, and allele appendix |
🔬 What this test provides: High‑resolution HLA typing at allele‑level resolution for Class I (A, B, C) and Class II (DRB1, DQB1, DPB1) loci using Illumina sequencing. The report includes unambiguous G‑Codes (NMDP compatible), segment sequencing data of exons 2–3 (Class I) and exon 2 (Class II), and an exhaustive appendix of detected alleles with database version 3.51.0. Used for transplant matching, ankylosing spondylitis association (HLA‑B27), and drug hypersensitivity screening.
High Resolution HLA Typing Test for Single Person: ₹8,000 | High‑Resolution NGS Report with G‑Codes
Clinically Validated by Dr. Ramachandra Pendurti (Reg No: 3585), General Physician. Reporting complies with 2026 International Histocompatibility and Immunogenetics (IHI) standards.
📄 Report Deep‑Dive: What You Receive
Unlike conventional low‑resolution HLA typing (PCR‑SSP) that leaves allele ambiguity, our NGS‑based report delivers protein‑level accuracy across the most clinically relevant exons:
- Class I loci (A, B, C): Full segment sequencing of exon 2 and exon 3 – the regions encoding the peptide‑binding groove.
- Class II loci (DRB1, DQB1, DPB1): High‑depth sequencing of exon 2 (the hypervariable domain).
- Resolution: Allele‑level (four‑digit) with unambiguous G‑Codes (Group Codes as per NMDP).
- Data presentation: Each allele is reported with its official NMDP allele code, G‑Code, and the specific nucleotide variations detected.
- Appendix of included alleles: The report includes a full list of all alleles covered by the assay, with reference to the Allele Database Version 3.51.0 (IPD‑IMGT/HLA).
- Interpretation guide: For disease association (e.g., HLA‑B27 for ankylosing spondylitis) and transplant compatibility (match grade).
🧬 Methodology: Illumina Next‑Generation Sequencing (NGS)
We use Illumina sequencing chemistry with locus‑specific long‑range PCR amplification. This method sequences both alleles simultaneously, resolving phase ambiguity that plagues Sanger or SSP methods. The average read depth exceeds 500× per exon, ensuring confidence in variant calling. The final genotype is assigned using dedicated HLA typing software (e.g., OptiType or HLA‑LA) against the current IPD‑IMGT/HLA reference database.
📊 DNA Labs India NGS High Resolution HLA Typing vs. Hospital PCR‑SSP
| Feature | DNA Labs India (Illumina NGS) High Resolution | Standard Hospital PCR‑SSP |
|---|---|---|
| Resolution | Allele‑level (4‑digit) / G‑Codes | Low‑resolution (2‑digit) or intermediate |
| Ambiguity | Phase‑resolved – no ambiguous allele combinations | Frequent cis/trans ambiguities |
| Loci covered | A, B, C, DRB1, DQB1, DPB1 (6 loci) | Usually A, B, DRB1 only |
| Exon coverage | Class I: exons 2‑3; Class II: exon 2 | Limited to product‑size fragments |
| Clinical use | Transplant matching, disease risk, pharmacogenetics (e.g., abacavir hypersensitivity) | Basic donor matching only |
🩺 Why NGS is Preferred for High Resolution HLA Typing (2026 Standards)
Conventional methods (SSP, SSO) often produce ambiguous results because they cannot differentiate between alleles that differ only in phase (e.g., cis/trans). NGS resolves this by sequencing each allele individually. This is critical for:
- Unrelated donor transplantation – where a single mismatch at the allele level reduces graft survival.
- Disease association – e.g., HLA‑B27 for ankylosing spondylitis, HLA‑DQ2/DQ8 for celiac disease.
- Drug hypersensitivity – HLA‑B*57:01 for abacavir, HLA‑B*15:02 for carbamazepine (Stevens‑Johnson syndrome).
📋 Validated by Dr. Ramachandra Pendurti (Reg No: 3585)
This HLA typing protocol and reporting format have been reviewed against 2026 International Histocompatibility and Immunogenetics (IHI) standards. The use of G‑Codes and NMDP allele codes ensures compatibility with global transplant registries. Each report is cross‑checked with the IPD‑IMGT/HLA database version 3.51.0.
❓ Frequently Asked Questions – High Resolution HLA Typing
🔹 Why is NGS preferred over PCR‑SSP for HLA typing?
NGS eliminates allele ambiguity by sequencing each allele individually. SSP often yields multiple possible allele combinations, which is unacceptable for high‑stakes transplant matching. NGS also provides G‑Codes that are directly accepted by the NMDP (National Marrow Donor Program) registry.
🔹 How to read G‑Codes in my HLA report?
A G‑Code (e.g., “HLA‑A*01:01G”) represents a group of alleles that encode identical protein sequences for the relevant exons. It simplifies reporting while preserving clinical precision. The report includes a table mapping each G‑Code to the underlying alleles.
🔹 Is home collection available for HLA tests in Tier‑1 cities?
Yes. Free home sample collection is available in 300+ cities including all Tier‑1 (Mumbai, Delhi, Bangalore, Chennai, Kolkata, Hyderabad, Pune, Ahmedabad) and most Tier‑2 cities. A phlebotomist will collect whole blood in EDTA tubes and transport at 2‑8°C.
🔹 What diseases can HLA typing help diagnose or predict?
HLA typing is strongly associated with: ankylosing spondylitis (HLA‑B27), celiac disease (HLA‑DQ2/DQ8), type 1 diabetes (HLA‑DR3/DR4), rheumatoid arthritis (HLA‑DR4), and drug hypersensitivity (HLA‑B*57:01, B*15:02).
🔹 How long does it take to receive the report?
Turnaround time is 10‑12 working days from sample receipt at the lab, due to library preparation, sequencing, and bioinformatic analysis. An expedited option is available at additional cost.
📋 Book High Resolution HLA Typing (Single Person) – ₹8,000
| Test Name | HLA Typing – Single Person (6 Loci: A, B, C, DRB1, DQB1, DPB1) |
| Price | ₹10,000 (fixed for single individual; no hidden charges) |
| Home Collection | Free (300+ cities) |
| Report Delivery | PDF with G‑Codes, NMDP codes, allele appendix (version 3.51.0) |
📞 Call or WhatsApp: 09395142800 | Book Online
