Overview
Eukaryotic mRNA sequencing library preparation is a fundamental process in molecular biology that enables the comprehensive analysis of gene expression in eukaryotic organisms. This technique involves several critical steps: starting with the extraction of high-quality total RNA from biological samples, followed by the enrichment of polyadenylated mRNA molecules to focus on the coding transcriptome. The enriched mRNA is then fragmented to an optimal size, and reverse transcription is employed to synthesize complementary DNA (cDNA). Adaptors are ligated to the cDNA fragments, which are subsequently amplified through PCR to generate a sequencing-ready library. At DNA Labs India, we provide this service at a competitive cost of INR 11000, leveraging state-of-the-art Illumina sequencing platforms to ensure high accuracy and depth. Our experienced team guarantees meticulous library preparation, making it suitable for a wide range of applications such as disease diagnosis, including cancer and genetic disorders, as well as for research in developmental biology, personalized medicine, and biomarker discovery. The test facilitates the identification of differentially expressed genes, offering insights into disease mechanisms and potential therapeutic targets. With the convenience of free home sample collection available in numerous cities across India, we strive to deliver reliable and accessible diagnostic services to support your healthcare needs.
To be completed — reclassified from Tests on 2026-11-13.
The purpose of Eukaryotic mRNA Sequencing Library Preparation is to prepare high-quality sequencing libraries from mRNA samples for downstream analysis. This enables the identification of gene expression patterns, which is crucial for diagnosing diseases, understanding biological mechanisms, and developing targeted therapies.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: 1 week
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | 1 week | ₹11,000 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is Eukaryotic mRNA Sequencing Library Preparation?
It is a process to prepare sequencing libraries from mRNA for analyzing gene expression in eukaryotic cells.
How much does it cost at DNA Labs India?
The cost is INR 11000, including RNA extraction, library preparation, and sequencing.
What samples are required for this test?
Extracted RNA samples are required, with specific quality standards such as high RNA integrity.
How long does it take to get results?
Results are typically delivered within 1 week.
Is home sample collection available?
Yes, free home collection is available across many cities in India.
What diseases can this test help diagnose?
It can aid in diagnosing cancer, neurological disorders, and genetic diseases.
How is the library prepared?
Steps include RNA extraction, mRNA enrichment, fragmentation, cDNA synthesis, adaptor ligation, and PCR amplification.
What technology is used for sequencing?
We use Illumina sequencing technology for accurate results.
Are there any risks involved?
Risks are minimal, primarily related to sample handling and contamination.
How should I prepare for the test?
Ensure sample integrity by proper collection and storage; no fasting required.
What do the results mean?
Results show gene expression levels, which can indicate disease presence or research insights.
Can it be used for research purposes?
Yes, it is suitable for research on gene expression, biomarkers, and disease mechanisms.
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