Overview
Eukaryotic stranded transcriptome library preparation is a sophisticated molecular biology technique used to convert RNA into a complementary DNA (cDNA) library for sequencing. This method specifically separates the sense and antisense strands of RNA, enabling researchers to distinguish between them and identify non-coding RNAs, such as long non-coding RNAs (lncRNAs). At DNA Labs India, we offer this service to support advanced genomic research. The process involves high-quality RNA extraction, cDNA synthesis with strand-specific adapters, library amplification, and next-generation sequencing. This approach provides several benefits, including the ability to uncover non-coding RNAs that play crucial roles in gene regulation and disease mechanisms. It also improves sequencing accuracy by reducing errors associated with strand ambiguity, leading to a more comprehensive understanding of gene expression. Our service includes library preparation, sequencing, and basic data analysis, all at a competitive cost of INR 16,500. This test is ideal for researchers studying eukaryotic gene expression, transcriptomics, and functional genomics. By leveraging stranded transcriptome analysis, scientists can gain deeper insights into cellular processes and identify potential biomarkers for various conditions.
To be completed — reclassified from Tests on 2026-11-13.
To prepare a strand-specific cDNA library from eukaryotic RNA for sequencing, enabling detailed analysis of gene expression, identification of non-coding RNAs like lncRNAs, and research into transcriptomic regulation.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: 1 week
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | 1 week | ₹16,500 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is eukaryotic stranded transcriptome library preparation?
It is a method to convert RNA into a cDNA library for sequencing while preserving strand information, allowing identification of both coding and non-coding RNAs.
Why is strand separation important in RNA sequencing?
Strand separation helps distinguish between sense and antisense transcripts, improving accuracy and enabling detection of non-coding RNAs like lncRNAs.
What are lncRNAs and why are they important?
Long non-coding RNAs (lncRNAs) are RNA molecules that do not code for proteins but regulate gene expression and are implicated in various diseases.
How much does this test cost at DNA Labs India?
The cost is INR 16,500, which includes library preparation, sequencing, and basic data analysis.
What sample type is required for this test?
Extracted RNA of high quality (RIN >7) is required, typically from eukaryotic sources.
How long does it take to get the results?
Results are typically delivered within 1 week via online portal, email, or WhatsApp.
Is home sample collection available?
Yes, free home sample collection is available for online bookings across India.
What are the benefits of this test over non-stranded methods?
Benefits include improved accuracy, ability to identify non-coding RNAs, and a more comprehensive view of gene expression.
Can this test be used for diagnostic purposes?
No, this test is intended for research use only and not for clinical diagnosis.
What is the turnaround time for the report?
The turnaround time is 1 week from sample receipt.
How should I prepare the sample for this test?
Ensure RNA is extracted using RNase-free methods, stored at -80°C, and shipped on dry ice to prevent degradation.
Is this test covered by insurance?
Generally, this research test is not covered by insurance; check with your provider for specific funding options.
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