Overview
Eukaryotic transcriptome library preparation is a fundamental technique in molecular biology used to analyze gene expression patterns in eukaryotic organisms. This process involves converting RNA molecules into complementary DNA (cDNA) fragments that can be sequenced using next-generation sequencing technologies. The library preparation includes several critical steps: RNA extraction from samples such as tissues or cell cultures, quality assessment to ensure RNA integrity, fragmentation of RNA into smaller pieces, synthesis of cDNA via reverse transcription, addition of sequencing adapters, and amplification through PCR. High-quality libraries are essential for accurate sequencing and downstream analysis. This test is particularly important for studying complex gene structures and regulatory mechanisms in eukaryotes, enabling researchers to discover novel transcripts like long non-coding RNAs (lncRNAs), which play roles in various biological processes and diseases. Applications span gene expression analysis under different conditions, functional annotation of genes, and biomarker discovery. The cost for this service is INR 15000 per sample, with free home collection available across India. Quality control at each step is crucial to diagnose and prevent issues such as low RNA quality or poor library efficiency.
To be completed — reclassified from Tests on 2026-11-13.
The purpose of eukaryotic transcriptome library preparation is to enable comprehensive gene expression analysis by converting RNA into sequenceable cDNA libraries. This allows for the identification of novel transcripts, including lncRNAs, functional annotation of genes, and insights into biological processes and disease mechanisms.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: 1 week
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | 1 week | ₹15,000 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is eukaryotic transcriptome library preparation?
It is a process to convert RNA from eukaryotic cells into cDNA libraries for sequencing, enabling gene expression analysis.
Why is this test important for gene expression analysis?
It allows researchers to study complex gene structures and regulatory mechanisms, identifying novel transcripts like lncRNAs.
What samples are required for this test?
Extracted DNA or tissue/cell culture samples, with RNA extraction performed as part of the process.
How long does it take to get results?
Results are typically available within 1 week after sample collection.
What is the cost of the test?
The cost is INR 15000 per sample, with free home collection available across India.
Is home sample collection available?
Yes, free home sample collection is offered for online bookings in numerous cities across India.
What are the applications of this test?
Applications include gene expression analysis, discovery of lncRNAs, functional annotation of genes, and biomarker discovery.
How is RNA quality assessed?
RNA quality is assessed using metrics like RNA Integrity Number (RIN), with a value >7.0 indicating high quality.
What are common problems in library preparation?
Common issues include low RNA quality, poor library yield, and adapter contamination, which can be diagnosed through quality control checks.
Can this test detect lncRNAs?
Yes, it is specifically designed to include the detection and analysis of long non-coding RNAs (lncRNAs).
Is the test covered by insurance?
Coverage depends on the insurance provider and policy; it is not typically covered under standard schemes like PMJAY or CGHS.
How should I prepare for the test?
Ensure sample integrity by following collection instructions, and provide any relevant medical history for accurate analysis.
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