Overview
Sequencing Library QC-qPCR is a vital quality control step in next-generation sequencing (NGS) workflows. It accurately quantifies the DNA library concentration and assesses its quality using quantitative PCR (qPCR). This ensures that the library is free from contaminants, inhibitors, and adapter dimers that could compromise sequencing accuracy. At DNA Labs India, we offer this essential service at an affordable cost of INR 2800, with free home sample collection across major cities in India. Our state-of-the-art facility and experienced geneticists ensure reliable results, helping researchers and clinicians achieve high-quality sequencing data. By identifying potential issues early, this test saves time and resources, preventing failed sequencing runs and rework. Whether you are preparing for whole-genome sequencing, targeted panels, or RNA-seq, our QC-qPCR service is your first step toward accurate and reproducible results.
To be completed — reclassified from Tests on 2026-11-13.
The primary purpose of Sequencing Library QC-qPCR is to determine the concentration of the DNA library and verify its quality before sequencing. This is crucial because accurate quantification ensures optimal cluster density on the flow cell, leading to high-quality sequencing data. The test also detects the presence of contaminants or inhibitors that could interfere with sequencing enzymes, thereby preventing failed runs and reducing costs. By providing precise measurements, this QC step enables researchers to make informed decisions about library dilution and pooling, ensuring efficient use of sequencing resources.
Sample requirements
What to send us
Deliverables & turnaround
What you receive
Turnaround: Reports are typically delivered within 1 week after sample collection. The turnaround time may vary depending on the location and sample condition.
Pricing
Transparent pricing
| Option | Turnaround | Price (INR) | Unit |
|---|---|---|---|
| Standard | Reports are typically delivered within 1... | ₹2,800 | per sample |
Limitations & precautions
What this service cannot do
Questions customers ask
Frequently asked questions
What is Sequencing Library QC-qPCR?
Sequencing Library QC-qPCR is a quality control test that uses quantitative PCR to measure the concentration and quality of a DNA library before next-generation sequencing. It ensures the library is free from contaminants and inhibitors, and has the correct concentration for optimal sequencing results.
Why is library QC important before sequencing?
Library QC is crucial because it ensures that the DNA library is of sufficient quality and quantity for sequencing. Poor quality libraries can lead to failed sequencing runs, low data output, and inaccurate results. QC helps identify issues early, saving time and money.
What sample is required for this test?
The sample required is extracted DNA. The DNA should be of good quality and quantity. The lab will provide instructions on how to prepare and send the sample.
How much does the Sequencing Library QC-qPCR cost?
The test costs INR 2800. This includes home sample collection and the qPCR assay. There are no hidden charges.
How long does it take to get the results?
The turnaround time is typically 1 week. Reports are delivered via online portal, email, or WhatsApp.
Is fasting required for this test?
No, fasting is not required for this test.
Can I get a home sample collection?
Yes, DNA Labs India offers free home sample collection for online bookings. This service is available in many cities across India.
What does the test detect?
The test detects the concentration of the DNA library and checks for the presence of contaminants, inhibitors, and adapter dimers that could affect sequencing.
Who should get this test?
This test is recommended for researchers and clinicians who are preparing DNA libraries for NGS. It is also useful for anyone who has experienced sequencing failures and wants to troubleshoot the issue.
What is the difference between qPCR and other quantification methods?
qPCR is more accurate than UV spectrophotometry or fluorometry because it specifically amplifies the library molecules and detects inhibitors. It provides a functional quantification that correlates with sequencing performance.
Are there any risks associated with this test?
The test itself is non-invasive and has no risks. The only risk is from the sample collection procedure (e.g., blood draw), which may cause minor bruising or infection, but this is rare.
How should I prepare my DNA sample for the test?
Ensure that the DNA is extracted using a reliable method and is free from contaminants. The sample should be stored at -20°C or -80°C and shipped on dry ice if necessary. Follow the lab's instructions for sample submission.
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